Identification of microsatellite loci, gene ontology and functional gene annotations in Indian salmon (Eleutheronema tetradactylum) through next-generation sequencing technology using illumina platform

An Introduction to Illumina Next-Generation Sequencing Technology for Microbiologists

An Introduction to Illumina Next-Generation Sequencing Technology for Microbiologists

Deciphering DNA sequences is essential for virtually all branches of biological research. Capillary electrophoresis (CE)-based sequencing has enabled scientists to elucidate genetic information from almost any organism or biological system. Although this technology has become widely adopted, inherent limitations in throughput, scalability, cost, speed, and resolution can hinder scientists from ...

Applicability of Next Generation Sequencing Technology in Microsatellite Instability Testing

Microsatellite instability (MSI) is a useful marker for risk assessment, prediction of chemotherapy responsiveness and prognosis in patients with colorectal cancer. Here, we describe a next generation sequencing approach for MSI testing using the MiSeq platform. Different from other MSI capturing strategies that are based on targeted gene capture, we utilize "deep resequencing", where we focus ...

Development and characterization of 26 novel microsatellite loci for the trochid gastropod Gibbula divaricata (Linnaeus, 1758), using Illumina MiSeq next generation sequencing technology.

In the present study we used the high-throughput sequencing technology Illumina MiSeq to develop 26 polymorphic microsatellite loci for the marine snail Gibbula divaricata. Four to 32 alleles were detected per locus across 30 samples analyzed. Observed and expected heterozygosities ranged from 0.130 to 0.933 and from 0.294 to 0.956, respectively. No significant linkage disequilibrium existed. S...

Defining functional distance using manifold embeddings of gene ontology annotations.

Although rigorous measures of similarity for sequence and structure are now well established, the problem of defining functional relationships has been particularly daunting. Here, we present several manifold embedding techniques to compute distances between Gene Ontology (GO) functional annotations and consequently estimate functional distances between protein domains. To evaluate accuracy, we...